Sony Digital Rapids Pixel Power calrec Sony

August 07, 2018

09/08/2018

New antibody analysis accelerates rational vaccine design Scripps Research study illuminates humoral immune response to experimental vaccines.

August 07, 2018

A team at Scripps Research has come up with a faster way to analyze the outcome of experimental vaccines against HIV and other pathogens. Their new system lets scientists quickly assess the full spectrum of antibodies produced in an individual in response to a pathogen or vaccine and determine if these antibodies are likely to be effective against the pathogen.

We can now watch antibody responses evolve almost in real time, says Lars Hangartner, PhD, a Scripps Research associate professor and co-senior author of the study, which published today in the journal Immunity.

This can be applied to any pathogen, adds Andrew Ward, PhD, professor at Scripps Research and study co-senior author. Ward is also affiliated with the International AIDS Vaccine Initiative (IAVI) Neutralizing Antibody Center and Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery (CHAVI-ID) at Scripps Research.

When a pathogen attacks, your immune system amasses an army of antibodies to fight back. Some of these antibodies will fail, but some may get close to beating a pathogen's defenses. Over time, the best antibodies will evolve to target a pathogen's vulnerable sites. If all goes well, antibodies will home in on those epitopes, neutralize the invader and provide lifelong immunity.

Vaccines work by tricking the body into producing neutralizing antibodies against a future invader. Testing vaccines tends to be a lengthy process, with an initial immunization and then a series of booster shots over weeks or months. The time it takes to analyze the trial data means scientists don't usually get to see if a vaccine is working until the experiment is over, making it especially hard to tweak treatment strategies in the face of emerging diseases.

With the new study, scientists finally have a way to get a nearly real-time picture of antibody evolution. The new technique builds on Scripps Research-led breakthroughs in immunology and an imaging technique called electron microscopy, which reveals the structures of antibodies bound to their target pathogens, such as HIV.

The researchers used rabbit blood samples collected at different stages of an HIV vaccine trial. The researchers purified the samples, breaking down the molecules until they could extract antibody fragments. They then mixed these antibody fragments with their targets viral proteins and imaged them in the electron microscope, revealing how the immune system was developing its attack on the pathogen.

After fine-tuning the biochemistry behind the formation of immune complexes of HIV Env trimers with antibody fragments, we were able to establish a very solid protocol allowing for semi-quantitative electron microscopy analysis, says study co-first author Matteo Bianchi, PhD, a former Scripps researcher now with the Institute of Medical Virology, University of Zurich.

This new method is based on images taken by a relatively low-tech method called negative stain imaging, which helped them spot promising, or more often than not, distracting, antibodies. At last, the researchers could quickly find out if a vaccine was pushing the immune system along the right path. And in cases where a vaccine wasn't working, the method could provide researchers with information as to how the vaccine could be improved.

You can change the direction of a study depending on the results from this method, says study co-first author Hannah Turner, a research assistant in the Ward lab. Never before have we been able to take a snapshot of our antibodies like this giving us a more complete picture of what is happening and how we can influence the vaccination process.

While the images were not high-resolution, the researchers were excited to report that this level of analysis which only takes days could become a routine test around the world. Most institutes have at least the technology and resources to do this level of analysis, says Ward.

For an even closer look at the antibodies, Ward used higher-tech cryo-electron microscopy to get high-resolution, 3-D images of the antibodies with their viral targets. These detailed snapshots revealed additional details that may help scientists improve future experimental HIV vaccines.

The researchers say their faster method could open the door to personalized design for vaccines. For example, this study included a high-responder rabbit that produced many effective antibodies, and a low-responder rabbit with a weak army of antibodies. Going forward, researchers could use this new method in human vaccine or therapeutic trials to quickly separate high responders from low responders early on, saving crucial time for patients and their doctors as they search for effective therapies and vaccines that provide herd immunity.

This is going to be part of every immunization project I do from now on, Ward says.

This method has the potential to change the pace at which we can develop vaccines, adds Hangartner.

Additional authors of the study, Rapid mapping of polyclonal antibody responses by electron microscopy, were co-first authors Matteo Bianchi and Hannah Turner of Scripps Research; Bartek Nogal, Christopher Cottrell, David Oyen, Matthias Pauthner, Raiza Bastidas and Rebecca Nedellec of Scripps Research; Laura McCoy of University College London; Ian Wilson of Scripps Research, the International AIDS Vaccine Initiative-Neutralizing Antibody Center and the Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery; and Dennis Burton of Scripps Research, the International AIDS Vaccine Initiative-Neutralizing Antibody Center, the Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery; and the Ragon Institute.

The study was supported by the N
LINK: https://www.scripps.edu/news-and-events/press-room/2018/20180807-antib...
See more stories from scripps

Most recent headlines

24/01/2019

Swansea City AFC Launches Live Streaming Platform with URSA Broadcast

Swansea City AFC Launches Live Streaming Platform with URSA Broadcast posted: 23/01/2019 Blackmagic Design today announced that Swansea City Association F...

24/01/2019

Salamanders Announce Kevin Soine as Head Coach for 2019

Kevin Soine The Holly Springs Salamanders have announced Kevin Soine as the head coach for the 2019 season. Soine joins the Salamanders after spending two se...

24/01/2019

January 23, 2019

Humans may be capable of producing a quintillion different antibodies January 23, 2019 LA JOLLA, CA An analysis by Scripps Research scientists of billions ...

24/01/2019

USA Renews Suits' For Ninth and Final Season

USA Network has renewed lawyer drama Suits for a ninth season comprised of ten episodes. That will represent the final season of the Aaron Korsh drama. Suits p...

24/01/2019

Fosse/Verdon' Limited Series Starts on FX April 9

FX limited series Fosse/Verdon, about the romantic and creative partnership shared by Bob Fosse and Gwen Verdon, starts April 9. There are eight episodes. The s...

24/01/2019

Fox Nation's Sean Hannity Program Starts Jan. 30

Fox Nation, the new subscription streaming service from Fox News, debuts the Sean Hannity weekday show Hannity On Air Jan. 30. Hannity On Air will feature a liv...

24/01/2019

Syndication Ratings: Ratings Wake Up After Short Winters Nap

Syndication came back to life in the week ended Jan. 13, following two slow weeks that included the Christmas and New Year's holiday weeks that were marked ...

24/01/2019

Matt Okine's Stan original comedy series The Other Guy renewed for second season

23 01 2019 - Media release Matt Okine's Stan original comedy series The Othe...

24/01/2019

New hires to help push...

[L-R] Cyril Matter, Ramy Alam and Rosario Marseglia Collaboration company Mersive Technologies has bolstered its EMEA team with two regional sales managers and...

24/01/2019

Sony to move European HQ to the Netherlands to...

Sony Center HQ building in Tokyo / Shutterstock.com Sony will move its European headquarters from Britain to the Netherlands to avoid disruption caused by Brex...

24/01/2019

As Silicon Valley investment plummets, what's the future for...

Enthusiasm for VR is waning in Silicon Valley with the Los Angeles Times reporting a crash in investment and suggesting a wider slump in take up of the emerging...

24/01/2019

GSMA Announces Nominees for 2019 GLOMO Awards

London: The GSMA today announced the nominees for the Global Mobile Awards 2019 (GLOMO Awards). Winners of the awards will be presented throughout MWC19 Barcelo...

24/01/2019

CORE BY DPA - AS ADVERTISED

By Gy rgy Rajna, Production Sound Mixer and DPA Master As a motion picture sound professional, I am an everyday user of the DPA d:screet Miniature Microphones...

24/01/2019

ZEISS Xradia 610&620 Versa: ZEISS introduces next generation X-ray microscopes

JENA/Germany, 23.01.2019. ZEISS introduces two new advanced models of the ZEISS Xradia Versa family: The ZEISS Xradia 610 & 620 Versa X-ray microscopes excel th...

23/01/2019

VIZ MEDIA ACQUIRES HOME MEDIA AND PUBLISHING RIGHTS FOR POKMON THE MOVIE: THE POWER OF US

VIZ MEDIA ACQUIRES HOME MEDIA AND PUBLISHING RIGHTS FOR POK MON THE MOVIE: THE P...

23/01/2019

IMT Vislink Presents Newsnet All-IP ENG Solution at Ohio Association of Broadcasters Conference

Jan 22 IMT Vislink Presents Newsnet All-IP ENG Solution at Ohio Association of...

23/01/2019

Harvard Dual Degree Students Make Music on Both Sides of the River

Adjusting to university life is a challenge for any entering student. But for a handful of students enrolled in Berklee's dual degree program with Harvard U...

23/01/2019

Three Berklee Alumni Score Oscar Nods

Berklee alumni Gillian Welch '92 and David Rawlings '92 received their first Oscar nominations Tuesday morning for Best Original Song. They composed Wh...

23/01/2019

Adobe Acquires Allegorithmic, the Leader in 3D Editing and Authoring for Gaming and Entertainment

SAN JOSE, Calif.--(BUSINESS WIRE)--Adobe (Nasdaq:ADBE) today announced that it h...

23/01/2019

Comics on Kickstarter Keep Hitting New Highs

Camilla Zhang, Comics Outreach Lead at Kickstarter. The Kickstarter Comics category had a massive year in 2018, with backers from around the world pledging a...

23/01/2019

Michael B. Jordan's Outlier Society Cements Partnership with Warner Bros. Pictures Group in First-Look Film Deal

Michael B. Jordan's Outlier Society Cements Partnership with Warner Bros. Pi...

23/01/2019

Lotto results: Mega roll-over jackpot up for grabs on Wednesday

Always gamble responsibly and play only within your means. Pick your numbers right and you could walk away with a whopping R19 million on Wednesday night's...

23/01/2019

Lotto and Lotto Plus results for Wednesday, 23 January 2019

Are you ready for the Lotto and Lotto Plus draw on Wednesday, 23 January? It's yet another chance to find out whether or not you're South Africa's l...

23/01/2019

Uzalo latest episodes and teasers for 23 25 January 2019 watch it here

In November 2018, Uzalo celebrated a milestone. After wrapping up the filming of season four, the show celebrated clocking over 10 million viewers. It's no...

23/01/2019

Watch: Uzalo latest episode, Wednesday 23 January 2019 [video]

In today's episode of Uzalo: Mastermind and Mxolisi confront each other about the status of their brotherhood. Nonka pushes for her family to do better. MaN...

23/01/2019

Watch: Generations The Legacy latest episode Wednesday, 23 January [video]

Tonight on Generations The Legacy: There is yet another hiccup in the jewellery heist. Young love leads to a first kiss Sphe knows there's a problem and de...

23/01/2019

Sunday movie: A Quiet Place what you need to know

Sunday evenings are perfect for horror movies. Get the popcorn ready, turn the lights down - perhaps even make a blanket fort, we're not here to judge - and...

23/01/2019

Pop Star Pabllo Vittar Gives Voice to Brazil's LGBTQ Community

On Saturday evening in early November, 10,000 Brazilians gathered at the Hopi Hari amusement park just outside S o Paulo and held their lighters aloft. It was P...

23/01/2019

Broadband TV News - Providers miss out by ignoring credential sharing

Providers miss out by ignoring credential sharing Via Broadband TV News - 20 December 2018 - What has been an effective strategy for OTT video providers to win ...

23/01/2019

Total Telecom Credential sharing is costing video providers millions in lost revenue

Credential sharing is costing video providers millions in lost revenue Via Total...

23/01/2019

4rfv Cartesian release report - How consumers access streaming video: The risks of credential sharing

Cartesian release report - How consumers access streaming video: The risks of cr...

23/01/2019

Videonet Cartesian claims service providers are missing out on streaming subscribers by ignoring credential sharing

Cartesian claims service providers are missing out on streaming subscribers by i...

23/01/2019

Invitation to submit proposals for the Fiction Slate

The National Film and Video Foundations invitation to submit proposals for the Fiction Slate The National Film and Video Foundation (NFVF) is an agency of the...

23/01/2019

Invitation to submit Documentary Proposals for The Next 25 Years of Democracy 2019

The National Film and Video Foundation Invitation to submit Documentary Proposal...

23/01/2019

SVG Regional Platinum Series Event at Sneaky Big Studios to Stream Live

SVG Regional Platinum Series Event at Sneaky Big Studios to Stream Live By SVG Staff Tuesday, January 22, 2019 - 12:01 pm Print This Story Story Highlight...

23/01/2019

Save the Date: SVG Venue Summit Travels to Little Caesars Arena in Detroit, June 26-27

Save the Date: SVG Venue Summit Travels to Little Caesars Arena in Detroit, June...

23/01/2019

Super Bowl LIII: CBS Sports Ramps Up for Big Audio

Super Bowl LIII: CBS Sports Ramps Up for Big Audio 180 mics, all-networked comms will be deployed at Mercedes-Benz Stadium By Dan Daley, Audio Editor Wednesd...

23/01/2019

SVG On Demand: Cloud and Virtualization Workshop - How the Cloud Will Power the MiLB Media Revolution

SVG On Demand: Cloud and Virtualization Workshop - How the Cloud Will Power the ...

23/01/2019

The SVG Podcast: Michael Connelly, SVP/EP, Fox Sports Regional Networks

The SVG Podcast: Michael Connelly, SVP/EP, Fox Sports Regional Networks Talking the latest in tech, production, and operations with the RSN exec By Brandon Cos...

23/01/2019

Check Out the SVG SportsTechLive Blog: On Site at Super Bowl LIII for All the Latest News from Atlanta

Super Bowl LIII: CBS Sports Ramps Up for Big Audio By Dan Daley, Audio Editor | ...

23/01/2019

Clear-Com Appoints New Broadcast Sales Manager for Germany

Newly created role underlines commitment to growth in key European region Clear-Com has appointed Alex Hemme as the company's first Regional Sales Manager...

23/01/2019

Google Announces Successful Programmatic TV Buying

Runs Local TV Campaigns with Inventory from WO Programmatic, More Effective and Faster to Buy Google just released its latest case study on programmatic TV buyi...

23/01/2019

WideOrbit and Mediaocean Partner to Improve Avails Responses, Proposals, and More

Today we're unveiling big news for ad buyers and broadcasters. We have partn...

23/01/2019

VEON reaffirms commitment to addressing strategic relationship with GTH

Amsterdam, 23 January 2019 - VEON Ltd. (NASDAQ and Euronext Amsterdam: VEON), a leading global provider of connectivity and internet services, announces that it...

23/01/2019

Jordan's Al Mamlaka TV Broadcast Infrastructure Controlled By Lawo VSM

Al Mamlaka TV, a 24/7 HD news channel and Jordan's first state-funded public service broadcaster, went on-air in July 2018, featuring a VSM solution for fac...

23/01/2019

How pay-TV operators can drive revenue with data

In The News, Press Releases, How pay-TV operators can drive revenue with data 23/01/2019 It's been more than a decade since Paul Davenport's seminal C...