Sony Digital Rapids Pixel Power calrec Sony

August 07, 2018

09/08/2018

New antibody analysis accelerates rational vaccine design Scripps Research study illuminates humoral immune response to experimental vaccines.

August 07, 2018

A team at Scripps Research has come up with a faster way to analyze the outcome of experimental vaccines against HIV and other pathogens. Their new system lets scientists quickly assess the full spectrum of antibodies produced in an individual in response to a pathogen or vaccine and determine if these antibodies are likely to be effective against the pathogen.

We can now watch antibody responses evolve almost in real time, says Lars Hangartner, PhD, a Scripps Research associate professor and co-senior author of the study, which published today in the journal Immunity.

This can be applied to any pathogen, adds Andrew Ward, PhD, professor at Scripps Research and study co-senior author. Ward is also affiliated with the International AIDS Vaccine Initiative (IAVI) Neutralizing Antibody Center and Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery (CHAVI-ID) at Scripps Research.

When a pathogen attacks, your immune system amasses an army of antibodies to fight back. Some of these antibodies will fail, but some may get close to beating a pathogen's defenses. Over time, the best antibodies will evolve to target a pathogen's vulnerable sites. If all goes well, antibodies will home in on those epitopes, neutralize the invader and provide lifelong immunity.

Vaccines work by tricking the body into producing neutralizing antibodies against a future invader. Testing vaccines tends to be a lengthy process, with an initial immunization and then a series of booster shots over weeks or months. The time it takes to analyze the trial data means scientists don't usually get to see if a vaccine is working until the experiment is over, making it especially hard to tweak treatment strategies in the face of emerging diseases.

With the new study, scientists finally have a way to get a nearly real-time picture of antibody evolution. The new technique builds on Scripps Research-led breakthroughs in immunology and an imaging technique called electron microscopy, which reveals the structures of antibodies bound to their target pathogens, such as HIV.

The researchers used rabbit blood samples collected at different stages of an HIV vaccine trial. The researchers purified the samples, breaking down the molecules until they could extract antibody fragments. They then mixed these antibody fragments with their targets viral proteins and imaged them in the electron microscope, revealing how the immune system was developing its attack on the pathogen.

After fine-tuning the biochemistry behind the formation of immune complexes of HIV Env trimers with antibody fragments, we were able to establish a very solid protocol allowing for semi-quantitative electron microscopy analysis, says study co-first author Matteo Bianchi, PhD, a former Scripps researcher now with the Institute of Medical Virology, University of Zurich.

This new method is based on images taken by a relatively low-tech method called negative stain imaging, which helped them spot promising, or more often than not, distracting, antibodies. At last, the researchers could quickly find out if a vaccine was pushing the immune system along the right path. And in cases where a vaccine wasn't working, the method could provide researchers with information as to how the vaccine could be improved.

You can change the direction of a study depending on the results from this method, says study co-first author Hannah Turner, a research assistant in the Ward lab. Never before have we been able to take a snapshot of our antibodies like this giving us a more complete picture of what is happening and how we can influence the vaccination process.

While the images were not high-resolution, the researchers were excited to report that this level of analysis which only takes days could become a routine test around the world. Most institutes have at least the technology and resources to do this level of analysis, says Ward.

For an even closer look at the antibodies, Ward used higher-tech cryo-electron microscopy to get high-resolution, 3-D images of the antibodies with their viral targets. These detailed snapshots revealed additional details that may help scientists improve future experimental HIV vaccines.

The researchers say their faster method could open the door to personalized design for vaccines. For example, this study included a high-responder rabbit that produced many effective antibodies, and a low-responder rabbit with a weak army of antibodies. Going forward, researchers could use this new method in human vaccine or therapeutic trials to quickly separate high responders from low responders early on, saving crucial time for patients and their doctors as they search for effective therapies and vaccines that provide herd immunity.

This is going to be part of every immunization project I do from now on, Ward says.

This method has the potential to change the pace at which we can develop vaccines, adds Hangartner.

Additional authors of the study, Rapid mapping of polyclonal antibody responses by electron microscopy, were co-first authors Matteo Bianchi and Hannah Turner of Scripps Research; Bartek Nogal, Christopher Cottrell, David Oyen, Matthias Pauthner, Raiza Bastidas and Rebecca Nedellec of Scripps Research; Laura McCoy of University College London; Ian Wilson of Scripps Research, the International AIDS Vaccine Initiative-Neutralizing Antibody Center and the Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery; and Dennis Burton of Scripps Research, the International AIDS Vaccine Initiative-Neutralizing Antibody Center, the Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery; and the Ragon Institute.

The study was supported by the N
LINK: https://www.scripps.edu/news-and-events/press-room/2018/20180807-antib...
See more stories from scripps

Most recent headlines

26/05/2019

Live From the Indy 500: BSI Puts Fans in the Driver's Seat, on the Hood

Live From the Indy 500: BSI Puts Fans in the Driver's Seat, on the Hood A majority of the field will be loaded with POV cams By Kristian Hernandez, Assista...

26/05/2019

Live From the Indy 500: IMS Productions Waves a Checkered Flag at Racing's Greatest Spectacle

Live From the Indy 500: IMS Productions Waves a Checkered Flag at Racing's G...

26/05/2019

Live From the Indy 500: NBC Sports Revs Its Engine in Inaugural Coverage

Live From the Indy 500: NBC Sports Revs Its Engine in Inaugural Coverage The broadcaster lauds the quality service, synergy, and partnership of IMS Productions ...

26/05/2019

Malawi election results: Tensions run high as vote recount ordered

A Malawi court on Saturday ordered the electoral board not to announce the 21 May presidential election results, following opposition complaints of vote-rigging...

26/05/2019

Incoming! More rain set to douse Cape Town, dam catchment areas

Relief for the drought-stricken Western Cape continues to come in the form of early-season rainfall with more rain forecast for the region on Monday. At last c...

26/05/2019

Lotto and Lotto Plus results for Saturday, 25 May 2019

Are you ready for the Lotto and Lotto Plus draw on Saturday, 25 May? It's yet another chance to find out whether or not you're South Africa's latest...

26/05/2019

Nedbank Cup champions mourn loss of rising star

Nedbank Cup champions TS Galaxy have confirmed the passing of one of their players Thembinkosi Mbamba, who lost his life in a car accident in the early hours of...

26/05/2019

NETFLIX ACQUIRES CANNES FILM FESTIVAL AWARD WINNERS ATLANTICS AND I LOST MY BODY

Grand Prix Winner Atlantics is Mati Diop's feature directorial debut Cannes Critics' Week Award Winner I Lost My Body from Xilam Animation is J r ...

25/05/2019

Cyril Ramaphosa's presidential inauguration in review

Standing in front of the thousands of South African citizens, African dignitaries and international guests, President Ramaphosa at his presidential inauguration...

25/05/2019

Super Rugby LIVE: Stormers vs Highlanders follow scores and updates!

Super Rugby LIVE: Stormers vs HighlandersOur live updates will appear below. They might take a little while to load on slower connections. Please be patient, we...

25/05/2019

Free daily horoscope, celeb gossip and lucky numbers for 25 May, 2019

CELEBRITIES BORN ON THIS DAY: Lauryn Hill, Frank Oz, Anne Heche, Tom T. Hall, Connie Sellecca, Dixie Carter, Robert Ludlum, Mike Myers. ARIES (March 21-April 1...

25/05/2019

These are the five most luxurious spas in the world [photos]

Self-care the buzzword of 2019. How do you fill your cup? We looked all over the globe to find the spas that will offer you the ultimate in self-indulgence. ...

25/05/2019

Primetime Ratings: ABC Takes it All with Farrah Fawcett Special

ABC and CBS tied in Thursdays primetime ratings with a 0.6 and a 3 share but ABC stood out with the This Is Farrah Fawcett special from 8 p.m. to 10 p.m. NBC ha...

25/05/2019

With 18.6 Million Simultaneous Viewers Streaming VIVO IPL, Hotstar Shatters Viewership Record Again

With 18.6 Million Simultaneous Viewers Streaming VIVO IPL, Hotstar Shatters View...

25/05/2019

Akamai to Gather Global Business, Developer and Technology Leaders and Innovators at its Edge World 2019 Conference

Akamai to Gather Global Business, Developer and Technology Leaders and Innovator...

25/05/2019

Rachel Riley to become My Famous Babysitter for W

LONDON - Friday 24th May, 2019: Rachel Riley is the first star name to join My Famous Babysitter (4x60), the newest factual entertainment commission for UKTVs p...

25/05/2019

PENGISIAN LENGKAP KEMPEN SEMPURNA SEADANYA' ASTRO SEPANJANG ...

PENGISIAN LENGKAP KEMPEN SEMPURNA SEADANYA' ASTRO SEPANJANG SYAWAL Date Released:24 May 2019 Share Tweet Media Pack PETIKAN KENYATAAN RASMI WAKIL A...

25/05/2019

Happy New Year from VIZ Media!

Happy New Year from VIZ Media!...

25/05/2019

VIZ MEDIA WELCOMES SUMMER WITH NEW ANIME AND MANGA RELEASES FOR JUNE

VIZ MEDIA WELCOMES SUMMER WITH NEW ANIME AND MANGA RELEASES FOR JUNE Catch The Home Media Debuts Of SAILOR MOON SAILOR STARS, MEGALOBOX And New Publishing Rele...

25/05/2019

Ratings Roundup: St. Louis Blues Breaks Cable TV Ratings Barrier With WCF Game 6 Win

Ratings Roundup: St. Louis Blues Breaks Cable TV Ratings Barrier With WCF Game 6...

25/05/2019

Inside BT Tower Network Hub for Last Day of the Premier League Season

Inside BT Tower network hub for last day of the Premier League season By Fergal Ringrose, Editorial Director, Europe Thursday, May 23, 2019 - 17:06 Print T...

25/05/2019

ICC Extends Sunset+Vine Partnership to 2023 With Award of New Host Production Contract

ICC extends S V partnership with award of new host production contract By Ferga...

25/05/2019

Sky Deutschland's Alessandro Reitano Takes Over as Chair of SVG Europe

Sky Deutschland's Alessandro Reitano takes over as chair of SVG Europe By Fergal Ringrose, Editorial Director, Europe Thursday, May 23, 2019 - 17:17 Pr...

25/05/2019

SportTech Takes Lord's by Storm With Three Events in One Day and Record Attendance

SportTech takes Lord's by storm with three events and record attendance By ...

25/05/2019

DAZN Boxing Stream Relies on Broadcast-Audio Basics

DAZN Boxing Stream Relies on Broadcast-Audio Basics Startup sports streamer eyes surround and immersive for the future By Dan Daley, Audio Editor Friday, May...

25/05/2019

NBC Sports Gears Up for Indy 500 Audio

NBC Sports Gears Up for Indy 500 Audio Two weeks of testing, practice runs, races helps the broadcaster fine-tune its strategy By Dan Daley, Audio Editor Fri...

25/05/2019

Lagardre announces the signing of the contract to sell its Television business to the M6 group under suspensive conditions

Lagard re announces the signing of the contract to sell its Television business ...

25/05/2019

ZACH GALIFIANAKIS STARS IN BETWEEN TWO FERNS: THE MOVIE PRODUCED BY FUNNY OR DIE TO BE RELEASED ON SEPTEMBER 20, 2019 ON NETFLIX

At this evening's FYSEE Event for My Next Guest Needs No Introduction With D...

24/05/2019

Fujifilm Europe is Innovator of the Year 2019

Fujifilm Europe is Innovator of the Year 2019 German industry publication Die Deutsche Wirtschaft has named Fujifilm Europe Innovator of the Year 2019 for its s...

24/05/2019

Sound Devices Closed for Memorial Day

Sound Devices is closed for Memorial Day, May 27th. We will reopen May 28th at 8:30 CST. To everyone observing the holiday have a wonderful weekend!...

24/05/2019

STARLINK MISSION

On Thursday, May 23 at 10:30 p.m. EDT SpaceX launched 60 Starlink satellites from Space Launch Complex 40 (SLC-40) at Cape Canaveral Air Force Station, Florida....

24/05/2019

Further Ofcom rules to support fibre investment

Further Ofcom rules to support fibre investment Add to Your Pages 24 May 2019 New rules give rival firms better access to Openreachs infrastructure M...

24/05/2019

KOBA Show 2019

CET ....Panther at KOBA Show 2019: we have an amazing show here in Seoul, South Korea. Popular products such as our Classic Plus dolly & U-Bangi-Slider are aro...

24/05/2019

Dolby Laboratories and Breast Cancer Prevention Partners Host LUNAFEST San Francisco

Back PDF Version May 24, 2019 Dolby Laboratories and Breast Cancer Preventi...

24/05/2019

ITV News Appoints Nina Hossain as Lead Presenter for ITV's Lunchtime News

ITV News has appointed Nina Hossain as lead presenter for ITV's Lunchtime News. Nina, who has won the prestigious RTS Nations and Regions Presenter award fo...

24/05/2019

Dolby Laboratories and Breast Cancer Prevention Partners Host LUNAFEST San Francisco

All Press Releases Back PDF Version May 24, 2019 Dolby Laboratories and B...

24/05/2019

First look at epic new ITV drama, Beecham House, coming to ITV this summer

Published: Fri 24 May 2019 Video of BeechamHouseITV First look at epic new ITV drama Beecham House coming to ITV this summer Watch and embed this video on y...

24/05/2019

Berklee to Present Wang Leehom Scholarship to Zhengxi Zhang

Berklee College of Music will present a four-year, full-tuition scholarship honoring Chinese-American singer-songwriter Wang Leehom '99 to Zhengxi Zhang, a ...

24/05/2019

AFP appoints photo journalist to top Mena

A former photo director for Latin America and then Europe-Africa, Sylvain Estibal is currently Mexico bureau chief. In this role, he coordinated coverage of the...

24/05/2019

METLIFE WORLDWIDE BENEFITS LANDS 5TH CONSECUTIVE AWARD WIN AT 2019 EMMAS

METLIFE WORLDWIDE BENEFITS LANDS 5TH CONSECUTIVE AWARD WIN AT 2019 EMMAS NEW YORK, May 24, 2019 MetLife Worldwide Benefits has been awarded Best Employee Be...

24/05/2019

End Boring MIDI Drum Parts!

I like anything that kickstarts creativity and gets you out of a rut-which is what this tip is all about. And, there's even a bonus tip about how to create ...

24/05/2019

VIEW 2019 Announces First Set Of Speakers

TORINO, ITALY - Dean DeBlois, writer, director and executive producer of this year's How to Train Your Dragon: The Hidden World, and Changhan (CH) Kim, CEO ...

24/05/2019

TED Talk: Digital Domain Delivers Realtime Digital Human

FARNHAM, UK - Digital human. Virtual character. Virtual assistant. VTuber. Terms fast evolving the mind-set for interactions of the digital age. Communicating w...

24/05/2019

disguise vx 4 Servers Deliver Ambitious Eurovision Visuals

disguise vx 4 Servers Deliver Ambitious Eurovision Visuals posted: 24/05/2019 The hotly-anticipated Eurovision 2019 spectacular landed in Tel Aviv in May ...

24/05/2019

Finding a Natural Rebel in Richard Ashcroft

Finding a Natural Rebel in Richard Ashcroft posted: 24/05/2019 Undoubtedly one of the most influential British singers of the last two decades, Richard As...

24/05/2019

Extron veteran Steve Somers named AVIXA 2019...

Recently retired Extron vice president of engineering, Steve Somers, has been announced as the winner of the 2019 Mackey Barron distinguished achievement award ...

24/05/2019

ETC appoints Dick Titus...

Former ETC COO, Dick Titus, has been appointed as president/CEO of ETC. Titus started the company in 1990, having led the management team at ETC for 17 years ...

24/05/2019

AGB Events announce international expansion plans

AGB Events has announced plans to expand its operations, continuing to work with partnership with its network of AV suppliers. Working almost exclusively in A...

24/05/2019

Open offices cause hour...

A study commissioned by Poly has found that three quarters of office workers are regularly distracted at their personal workplace, with 36% of workers saying no...