JENA/Germany, WASHINGTON D.C./USA, 14.11.2014.
At the annual Neuroscience meeting in Washington, D.C., November 15-19, 2014, ZEISS will present new microscope hard- and software for imaging of cleared tissue.
Clearing methods make tissue transparent, thus allowing scientists to image deep into large biological samples such as tissue sections, brains, embryos, organs, spheroids or biopsies. The enhanced optical penetration depth even allows detection of fluorescent signals from whole organs. This means clearing is a promising technique when, for example, investigating neuronal networks in mouse brains.
The microscope system ZEISS Lightsheet Z.1 combines the advantages of clearing with light sheet fluorescence microscopy. Researchers are now able to image large cleared specimens with high light efficiency, speed, and almost no photo damage. ZEISS Lightsheet Z.1 acquires multiple tiles of Z-stacks with several thousand high quality images. A typical imaging speed of 10 to 40 frames per second reduces the imaging time from hours to minutes.
ZEISS now offers a range of objectives with long working distances, adapted to the refractive index of respective clearing media. In addition, the sample holder of ZEISS Lightsheet Z.1 is now ready for the special requirements of large and cleared samples.
3D imaging of large samples produces datasets in the Terabyte range that need to be stored, transported, viewed, and analyzed. ZEISS has worked together with arivis AG to provide a software platform that addresses these needs during the complete workflow. The imaging platform arivis Vision4D is a modular software package for fast visualization of 2D, 3D and 4D images of nearly unlimited size independent of RAM.
Thy1-EGFP M-line mouse hippocampus, optically cleared in LUMOS
clearing agent, imaged with ZEISS Lightsheet Z.1, data processing and 3D rendering in arivis Vision4D software.
Sample courtesy of O. Efimova, National Research Center Kurchatov Institute, Moscow, Russia
Dr. Jochen Tham
Carl Zeiss Microscopy GmbH
Phone: +49 3641 64-3949
Fax:
jochen.tham@zeiss.com
About ZEISS
ZEISS is an international leader in the fields of optics and optoelectronics. The more than 24,000 employees of ZEISS generated revenue of about 4.2 billion euros in fiscal year 2012/13. Founded in 1846 in Jena, the company is headquartered in Oberkochen, Germany. ZEISS has been contributing to technological progress for more than 160 years. ZEISS develops and produces solutions for the semiconductor, automotive and mechanical engineering industries, biomedical research and medical technology, as well as eyeglass lenses, camera and cine lenses, binoculars and planetariums. ZEISS is present in over 40 countries around the globe with more than 40 production facilities, around 50 sales and service locations and over 20 research and development sites. Carl Zeiss AG is fully owned by the Carl-Zeiss-Stiftung (Carl Zeiss Foundation).
The Microscopy Business Group
The Microscopy business group is the worlds only one-stop manufacturer of light, X-ray and electron microscopes. Its extensive portfolio enables research and routine applications in the life and materials sciences. The Microscopy business group is headquartered in Jena. Additional production and development sites are located in Oberkochen, G ttingen and Munich, as well as in Cambridge in the UK, in Peabody, MA and Pleasanton, CA in the USA. The business group has around 3,000 employees. It generated revenue of 629 million euros in fiscal year 2012/13.
Number: 0139-2014-ENG MI
Number of Words: 355
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JENA/Germany, WASHINGTON D.C./USA, 14.11.2014. 
