Sony Pixel Power calrec Sony

May 20, 2024

21/05/2024

New method to reveal what drives brain diseases Scripps Research scientists develop CRISPR screen technology to determine disease mechanism from tissues with accelerated speed.

May 20, 2024

LA JOLLA, CA The brain is often referred to as a black box one that's difficult to peer inside and determine what's happening at any given moment. This is part of the reason why it's difficult to understand the complex interplay of molecules, cells and genes that underly neurological disorders. But a new CRISPR screen method developed at Scripps Research has the potential to uncover new therapeutic targets and treatments for these conditions.

The method, outlined in a study published in Cell on May 20, 2024, provides a way to rapidly examine the brain cell types linked to key developmental genes at a scale never done before helping unravel the genetic and cellular drivers of different neurological diseases.

We know that certain genetic variants in our genome can make us vulnerable or resilient towards different diseases, but which specific cell types are behind a disease? Which brain regions are susceptible to the genome mutations in those cells? These are the kinds of questions were trying to answer, says senior author Xin Jin, PhD, an assistant professor in the Department of Neuroscience at Scripps Research. With this new technology, we want to build a more dynamic picture across brain region, across cell type, across the timing of disease development, and really start understanding how the disease happened and how to design interventions.

Thanks to over a decade's efforts in human genetics, scientists have had access to long lists of genetic changes that contribute to a range of human illnesses, but knowing how a gene causes a disease is very different than knowing how to treat the illness itself. Every risk gene may impact one or several different cell types. Comprehending how those cell types and even individual cells impact a gene and affect disease progression is key to understanding how to ultimately treat that disease.

This is why Jin, along with the study's first author, Xinhe Zheng, a PhD candidate and the Frank J. Dixon Graduate Fellow at Scripps Research, co-invented the new technique, named in vivo Perturb-seq. This method leverages CRISPR-Cas9 technology and a readout, single-cell transcriptomic analysis, to measure its impact on a cell: one cell at a time. Using CRISPR-Cas9, scientists can make precise changes to the genome during brain development, and then closely study how those changes affect individual cells using single-cell transcriptomic analysis for thousands of cells in parallel.

Our new system can measure individual cells' response after genetic perturbations, meaning that we can paint a picture of whether certain cell types are more susceptible than others and react differently when a particular mutation happens, Jin says.

Previously, the method for introducing the genetic perturbations into the brain tissue was very slow, often taking days or even weeks, which created suboptimal conditions for studying gene functions related to neurodevelopment. But Jin's new screening method allows for rapid expression of perturbation agents in living cells within 48 hours meaning scientists can quickly see how specific genes function in different types of cells in a very short amount of time.

The method also enables a level of scalability that was previously impossible the research team was able to profile more than 30,000 cells in just one experiment, 10-20 times accelerated from the traditional approaches. In many of the brain regions they examined, such as the cerebellum, they were able to collect tens of thousands of cells that previous labeling methods could not reach.

In a pilot study using this new technology, Jin and her team's interest was piqued when they saw a genetic perturbation elicit different effects when perturbed in different cell types. This is important because those impacted cell types are the sites of action for particular diseases or genetic variants. Despite their smaller population representations, some low-abundant cell types may have a stronger impact than others by the genetic perturbation, and when we systematically look at other cell types across multiple genes, we see patterns. That's why single-cell resolution being able to study every cell and how each one behaves can offer us a systematic view, Jin says.

With her new technology in hand, Jin plans to apply it to better understand neuropsychiatric conditions and how certain cell types correspond with various brain regions. Moving forward, Jin says she's excited to see this type of technology applied to additional cell types in other organs in the body to better understand a wide range of diseases in terms of tissue, development and aging.

This work and the researchers involved were supported by funding from the Dorris Scholar Award, the Frank J. Dixon Fellowship, the Mark Pearson Endowed Fellowship, the Stanley Center for Psychiatric Research at the Broad Institute of MIT and Harvard, the Simons Foundation Autism Research Initiative (grant SFARI #736613), the National Institute of Health (grant R01HG012819), the Impetus grant, the One Mind Rising Star Award, the Klingenstein-Simons Fellowship, the Mathers Foundation, the Baxter Young Investigator Award, the Larry L. Hillblom Foundation, the Scripps Research Collaborative Innovation Fund, the Chan Zuckerberg Initiative, the Conrad Prebys Foundation, the Astera Institute, and James Fickle.

In addition to Jin, authors of the study, Massively parallel in vivo Perturb-seq reveals cell type-specific transcriptional networks in cortical development are Xinhe Zheng, Boli Wu, Yuejia Liu, Grace S. Clarke, Abdullah Ashiq, Joshua Park, Jiwen Li, Zhilin Wang, Tianbo Qi and Yunxiao Zhang of Scripps Resear
LINK: https://www.scripps.edu/news-and-events/press-room/2024/20240520-jin-b...
See more stories from scripps

Most recent headlines

04/08/2024

Dalet Appoints Santiago Solanas as CEO to Lead Next Era of Growth and Innovation

Dalet, a leading technology and service provider for media-rich organizations, is excited to announce Santiago Solanas as its new Chief Executive Officer (CEO)....

16/06/2024

Media Links Achieves a Global Milestone with Studio Trans...

Media Links, a manufacturer and pioneer in Media over IP transport technology, has succeeded in a world s first3 demonstration of PTP1 and STL2 using IP over mi...

16/06/2024

ServusTV And Ease Live Revolutionize the Viewing Experien...

Ease Live (an Evertz company) and Red Bull Media House-owned broadcaster ServusTV, are building on their successful launch of interactive streaming experiences ...

16/06/2024

CJP champions Living Wage initiative

CJP Broadcast Service Solutions Ltd has been accredited as a Living Wage Employer. This commitment will see everyone working at CJP receive a minimum hourly wag...

15/06/2024

What Exactly Is Dad Rock? 21 Songs that Explain the Totally Not Embarrassing Genre

What Exactly Is Dad Rock? 21 Songs that Explain the Totally Not Embarrassing Gen...

15/06/2024

Another Stephen King Book Headed for Series on MGM Plus

MGM Plus has ordered The Institute, a thriller based on a Stephen King novel, for a series. Ben Barnes and Mary-Louise Parker will star, and Jack Bender and Ben...

15/06/2024

Mike McClain, WOFL-WRBW Orlando GM, Sets Retirement, and Jeff Maloney Expands Role

With the retirement of Mike McClain as senior VP and general manager of WOFL-WRB...

15/06/2024

Jimmy Fallon, NBCUniversal Re-Up Deal To Host The Tonight Show'

Jimmy Fallon, host of The Tonight Show, has renewed his deal with NBCUniversal, extending his tenure through 2028. Fallon began hosting the show in 2014. Before...

15/06/2024

Tom Green Returns! Prime Video Green'-Lights 3 Projects

Prime Video has greenlighted three Tom Green projects coming out of the Amazon Connected Worlds event in Toronto. They include an unscripted series, a comedy sp...

15/06/2024

Tegna Ending Daily Blast Live' Effective Sept. 6

Tegna said it was ending production of Daily Blast Live, which has aired on Tegna stations since it was launched in 2017....

15/06/2024

Believe in Something Unconventional, Something Unexplored,' NVIDIA CEO Tells Caltech Grads

NVIDIA founder and CEO Jensen Huang on Friday encouraged Caltech graduates to pu...

14/06/2024

Sundance Ignite x Adobe Fellowship Announce 2024 Cohort

Program Uplifts Emerging Filmmakers Ages 18 to 25...

14/06/2024

Jennifer Connelly Becomes Her Own Worst Enemy in Bad Behaviour

PARK CITY, UTAH JANUARY 21: Actor Jennifer Connelly attends the 2023 Sundance Film Festival Bad Behaviour Premiere at The Ray Theatre on January 21, 2023 in...

14/06/2024

ZTransform Delivers New Media facilities for Midco Arena

SEATTLE ZTransform recently delivered AV and media facilities to Augustana University's highly anticipated Midco Arena. Despite challenging tie constraints,...

14/06/2024

Omdia: OLED Materials Market Will Return to Growth in 2024

LONDON A new report from Omdia finds that the global market for OLED materials is poised for a rebound after experiencing a deficit in recent years....

14/06/2024

ATSC Honors Sung-Ik Park and Ali Dernaika for Outstanding Leadership

WASHINGTON D.C. During the annual NextGen Broadcast Conference this week, the ATSC honored Dr. Sung-Ik Park of Korea's Electronics and Telecommunications Re...

14/06/2024

TVMonaco Relies on Riedel's Intercom Solutions

WUPPERTAL, Germany Riedel Communications has announced that TVMonaco, the first general-interest public television channel in Monaco, has selected Riedels Artis...

14/06/2024

Children's Digital Learning Facility REDEE Adopts Blackmagic Design Workflow for Streaming

Children's Digital Learning Facility REDEE Adopts Blackmagic Design Workflow...

14/06/2024

NUGEN Audio Releases NUtility Toolkit

NUGEN Audio Releases NUtility Toolkit Brie Clayton June 14, 2024 0 Comments NUGEN Audio unveils its new NUtility Toolkit, which comprises its SigMod, ...

14/06/2024

Ready for kick-off: RTL unveils one of most modern studios in Europe' for Euro 2024

Paul Hering, head of studio and field production at RTL Deutschland tells TVBEur...

14/06/2024

Elvis, JFK cinematographers among judges for FilmLight Colour Awards

This years awards include Emerging Talent, which celebrates the work of young colourists (age 18-35) in commercial and music video creation By Jenny Priestley ...

14/06/2024

Meet the product specialist, newsroom solutions

David Cardinale, product specialist, newsroom solutions at CGI tells TVBEurope about the importance of keeping skills up to date in the rapidly evolving media i...

14/06/2024

HBO Renews House of the Dragon for a Third Season

HBO has announced that its high profile original drama series House of the Dragon has been renewed for a third season....

14/06/2024

Pixotope Announces Native Support for Unreal Engine 5.4

OSLO Pixotope has announced the availability of Pixotope 24.1.0 and is reporting that the release includes the full integration of Unreal Engine 5.4, which open...

14/06/2024

Cineverse Passes 10M YouTube Sub Mark

LOS ANGELES Cineverse Corp. is reporting that its YouTube channels have passed the 10 million subscribers mark, thanks to the success of eight brands that have ...

14/06/2024

NBA Finals Game 3 on ABC and ESPN up 2% to 11.4M Viewers

Ratings for the NBA Finals continue to improve with the average audience for NBA Finals Game 3 on ABC and ESPN up two percent from last year's Game 3, accor...

14/06/2024

Clear-Com Announces Gen-IC Virtual Intercom is Now Ready...

Clear-Com is thrilled to announce that the highly anticipated Gen-IC Virtual Intercom, a revolutionary system combining the power of cloud production with Cle...

14/06/2024

Acronis Expands its Security Offering Beyond Endpoint Pro...

Acronis, a global leader in cybersecurity and data protection, is pleased to introduce Acronis XDR the newest addition to the company's security solution po...

14/06/2024

Veset AdWise Launched in AWS Marketplace

Veset has announced that Veset AdWise is now available in AWS Marketplace. Veset AdWise is the advanced, cloud-based solution for real-time, in-video advertisin...

14/06/2024

Ease Live and Bedrock Jointly Create the Interactive M6PL...

Bedrock, the leading European streaming tech company, and Ease Live, an Evertz company providing innovative interactive TV technology, announce their newly form...

14/06/2024

V-Nova Surpasses 1000 Patent Milestone in Media Technolog...

V-Nova, a leading provider of data compression solutions, video compression technology, XR technology, AI acceleration and parallel processing for a multitude o...

14/06/2024

Capitol Broadcasting Company Teams Up With Triveni Digita...

Triveni Digital today announced that Capitol Broadcasting Company owner and operator of WRAL-TV, WRAZ-TV, WILM-TV, and WNGT-TV in North Carolina has selecte...

14/06/2024

Riedel Communications Powers TVMonacos Inaugural Broadcas...

Riedel Communications today announced that TVMonaco, the first general-interest public television channel in Monaco, has selected Riedel s cutting-edge Artist i...

14/06/2024

Orange Polska Elevates Video Experiences With Viaccess-Or...

Viaccess-Orca (VO), a global leader providing OTT and TV platforms, content protection, and advanced data solutions, announced today that pay-TV operator Orange...

14/06/2024

Pixotope Announces Native Support for Unreal Engine 5-4 A...

Pixotope, the leading software platform for end-to-end real-time virtual production solutions, today announced the availability of Pixotope 24.1.0. This release...

14/06/2024

NUGEN Audio Releases NUtility Toolkit

NUGEN Audio unveils its new NUtility Toolkit, which comprises its SigMod, AB Assist, Jotter and Aligner plug-ins. Ideal for entry-level and professional audio p...

14/06/2024

Ross Video Powers up InfoComm 2024 with Advanced AV Solut...

Ross Video is excited to announce its participation in InfoComm 2024, held from June 12 to 14 at the Las Vegas Convention Center. This year, Ross Video is set t...

14/06/2024

CLEARHAVEN PARTNERS ACQUIRES ZIXI

Zixi, provider of the award-winning Software-Defined Video Platform (SDVP ) and the industry leader for enabling reliable, scalable and cost-efficient live vide...

14/06/2024

NMR Events Leverages R2 Integration with Beam Dynamics fo...

Beam Dynamics, leaders in technology lifecycle management, has implemented its Data Improvement Platform (DIP) at NMR Events, a major American industrial stagi...

14/06/2024

Tiffen Tech Day 2024 Stabilizer Expo and Steadicam Garret...

Tiffen welcomes the filmmaking community for its annual Tech Day '24 featuring the Stabilizer Expo, live equipment demos, and Tech Talks with renowned Opera...

14/06/2024

Cine Gear Expo Ready for Tech Panels and Networking at Wa...

Excitement is building for the opening of Cine Gear Expo at the Warner Bros. Studios in Burbank. This year's expo holds much to look forward to from technol...

14/06/2024

Applications Being Accepted for 2024 WRAL Azalea Celebration

The 38th Annual WRAL Azalea Celebration is now accepting applications from non-profits for the 2024 plant giveaway. Sponsored by WRAL-TV, MIX 101.5 WRAL-FM, FOX...

14/06/2024

State of Golf Now Open at ATC

Tee up a fun golf at American Tobacco. State of Golf, Downtown Durham's premier golf & sports simulator lounge opened on June 11, 2024. Get inspired by US O...

14/06/2024

Dr. Lenora Helm Hammonds Named Dean of the Professional Education Division

Dr. Lenora Helm Hammonds Named Dean of the Professional Education Division A renowned jazz vocalist, educator, and Berklee alumna, Hammonds is known for her i...

14/06/2024

Joey Chestnut, Takeru Kobayashi To Take Part in Live Hot Dog Contest on Netflix

Joey Chestnut is a scratch for the Nathan's Famous Fourth of July International Hot Dog-Eating Championship, but he and another speed-eating luminary, Taker...

14/06/2024

Paula Ebben Shifts to Mornings at WBZ Boston

Paula Ebben will anchor mornings with Chris Tanaka at WBZ Boston starting June 17. Ebben currently anchors evening news and anchored WBZ's morning news from...

14/06/2024

Reni Calister, Quanny Carr Get Key Roles at Rock'n Robin Productions

Rock'n Robin Productions, the production outfit run by Robin Roberts, has announced two executive hires. Reni Calister is the new chief content officer and ...

14/06/2024

The Proudest Refugee': How Veronica Miller Charts Her Own Path at NVIDIA

When she was five years old, Veronica Miller (n e Teklai) and her family left their homeland of Eritrea, in the Horn of Africa, to escape an ongoing war with Et...